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Freezing can cause the peptide molecules to aggregate irreversibly, meaning they clump together and cannot be separated again

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Among them, the glutamate produced by the decomposition of glutamine via GLS not only provides the raw material for the synthesis of GSH but is also further metabolized into α-ketoglutarate (α-KG) in the mitochondria under the action of glutamate dehydrogenase (GLUD1) or transaminases and then enters the tricarboxylic acid cycle to produce ATP [29]